Cleaning Up a Spill (Biosafety Cabinet): Biosafety Level 3

Remember to wear appropriate PPE and leave the biosafety cabinet on during
spill cleanup To begin, apply disinfectant to a towel and decontaminate and remove items from the area of the spill. Put the waste from the spill in a biohazard bag inside the cabinet. The biohazard bag should never be kept outside the cabinet. Put down the towel over the spill, to prevent aerosolization. Apply the disinfectant, in this case bleach over the towel and work from the outside to the inside. Wait the appropriate amount of time for the disinfectant to work. Next, wipe up the spill and any excess liquid with the towels. Then, apply more disinfectant to the spill area. Once again, wait the appropriate amount of time for the disinfectant to work. Wipe up the area one more time with the towels. Because bleach was used as the disinfectant, a sterile water rinse is used because bleach will corrode the stainless steel inside the cabinet.

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