Penn State Hershey Institute for Personalized Medicine: A Tour

[ Music ] >> Jim Broach: [Background Music] Hello. My name is Jim Broach. I’m the Director of the Penn State Hershey
Institute for Personalized Medicine. And I’d like to welcome you. We inaugurated the institute in January of
this year with the goal of bringing all the tools of molecular biology and genomics to
bear on patient disease onset. And on the response of patients to various
disease treatments. I’d like to take you on a tour of the institute. I want to show you the biorepository where
we store our samples, the Genomics Center where we do the analysis of these samples,
and the Bioinformatics Core, in which we correlate the information that we get from the genomics
with the patient records that we gather from the electronic medical records. So come this way. [ Music ] The first step of the process is consenting
patients collecting and preparing samples. >> To make personalized medicine work, we
need you to donate samples. This is a completely voluntary process where
we’ll ask for blood, saliva, or samples left over from procedures. After you’ve consented with us, that means
give us permission to use your samples and your information, we will store your samples
and your health information indefinitely. >> None of your personalized information will
be on the tube. >> Jim Broach: The Critical Core Facility
within the institute is the bio repository. >> After blood is processed into storage tubes,
it is placed into long-term storage in secure freezers. [ Background Noise ] Samples are barcode labeled, placed in storage
boxes, and frozen in ultra-low temperature freezers until analysis. [ Background Noise ] >> The first step in the genomic processing
is to extract DNA from each of the blood samples. >> This is the QIAsymphony DNA extraction
robot. It uses magnetic bead technology to isolate
DNA from samples such as saliva and whole blood. It’s a high throughput system that allows
us to process large numbers of samples, up to 96 at one time, giving us high-quality
results in a short period of time. We load samples and a reagent kit into the
machine. The robot takes over, moving the sample from
place to place through the process. Depositing DNA into tubes at the end of the
run. At this point, we can remove the tubes with
the DNA, and use them for further processing or put them in the freezer for long-term storage. >> Now that you’ve extracted the DNA, we now
need to fragment the DNA to prepare it for its genomic analysis. >> [Background Music] So at this point, we
take the extracted DNA and put it into this machine where we use focused sound waves to
shear the DNA into smaller pieces. We need to take it into smaller pieces so
we can move it to the next step where we can efficiently make a library of the DNA so we
can move it into the next generation sequencing. >> Now if you’ll follow me, we’ll walk over
to the Genomics Core. A second critical facility within the institute
is the Genomics Core. >> We then take that isolated DNA, and here
in Genome Sciences, prepare that DNA for genome sequencing. Using state-of-the-art technology like this
[inaudible], we can then sequence the entire 3,000,000,000 bases of a human genome. >> I hope you’ve enjoyed your tour. As you can see from the resources that we
have at the institute, we can obtain a detailed portrait of each and every patient. Feel free to explore additional information
on our website at www.med.psu.edu/ipm. Thank you for your time. [ Music ]

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